LOCUS       cloning_vector;        3417 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEX-PUC-3T DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    cloning vector; GST-fusion protein; GST-stuffer fusion protein
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Fukunaga R, Hunter T.
  TITLE     MNK1, a new MAP kinase-activated protein kinase, isolated by a novel
            expression screening method for identifying protein kinase 
            substrates
  JOURNAL   EMBO J. 16 (8), 1921-1933 (1997)
  PUBMED    9155018
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Fukunaga R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-1998) Rikiro Fukunaga, Osaka University Medical 
            School, Department of Genetics B-3; 2-2 Yamadaoka, Suita, Osaka 
            565-0871, Japan (E-mail:fukunaga@genetic.med.osaka-u.ac.jp, 
            Tel:81-6-879-3318, Fax:81-6-879-3319)
REFERENCE   3  (bases 1 to 3417)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3417)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1997"; volume: "16"; issue: "8"; pages: "1921-1933"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAY-1998) Rikiro Fukunaga, Osaka University Medical School, 
            Department of Genetics B-3"; volume: " 2-2 Yamadaoka, Suita, Osaka 
            565-0871, Japan (E-mail:fukunaga@genetic.med.osaka-u.ac.jp, 
            Tel:81-6-879-3318, Fax"; pages: "81-6-879-3319"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..140
                     /label=AmpR promoter
     CDS             141..998
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1172..1760
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2171..2199
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    2207..2223
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2246..2899
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2906..2923
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     misc_feature    2966..3385
                     /note="Stuffer:that the stuffer DNA is derived from the
                     420bp PvuII fragment(1802-2221) of the human G-CSF receptor
                     cDNA (Acc# No.M38025)"
ORIGIN
        1 gcggccgctc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
       61 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      241 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      301 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      361 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      421 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      481 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      541 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      601 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      661 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      721 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      781 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      841 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      901 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
      961 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1021 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1081 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1561 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1861 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1921 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1981 cgattcatta atgcagcctc gaggtctgca ccaatgcttc tggcgtcagg cagccatcgg
     2041 aagctgtggt atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc
     2101 actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg
     2161 aaatgagctg ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa
     2221 caatttcaca caggaaacag tattcatgtc ccctatacta ggttattgga aaattaaggg
     2281 ccttgtgcaa cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt
     2341 gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt
     2401 tcccaatctt ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat
     2461 acgttatata gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat
     2521 ttcaatgctt gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag
     2581 taaagacttt gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat
     2641 gttcgaagat cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga
     2701 cttcatgttg tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc
     2761 gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta
     2821 cttgaaatcc agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg
     2881 tggcgaccat cctccaaaat cggatctggt tccgcgtgga tccccgggaa tttccggtgg
     2941 tggtggtgga attggccctg caggcctgga aggcatcctc ctccatgatt gtgggcaccc
     3001 aggagcccag gctgctgtga gctgggtctg ggacacttgg ccagagggga ttcttcctgt
     3061 tggggctgca acagagccag gcagttccac agaggcaggt gagcaacagc aggaggccga
     3121 acaggcccag gatgatgtgt agctccgacc cctctggggt caaggtcatc agggtgagga
     3181 ctgtactgtt ggtggcccca gcctggctgg cagccatgag gtggatgtga tacagactgg
     3241 cgggctccag gccatggagg acaaagccac gggaggaggc attcaggatg gcggagaagg
     3301 actggttctg agcgttggtc cagaagatgg tgtagtgggt aagggggctc ttccccagct
     3361 cagggggctc aggcacccac tccaggcctg cagggccaat tctagatgac taagctt
//
